※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Flap endonuclease 1
Protein Synonyms/Alias
 FEN-1
Gene Name
 fen
Gene Synonyms/Alias
 SSO0179
Created Date
 3-June-2014
Organism
 Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
NCBI Taxa ID
 273057
Phosphorylation
Position
Peptide
Code
Type
References
257 ALKIIKKYGKIEKAM Y HTP [1]
Reference
 [1]Change of carbon source causes dramatic effects in the phospho-proteome of the archaeon Sulfolobus solfataricus.
 Esser D,Pham TK,Reimann J,Albers SV,Siebers B,Wright PC
 J. Proteome Res. 2012, Oct, 5;11(10):4823-33. [PMID:22639831]
Functional Description From UniProt
 FUNCTION: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double- stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity)
Sequence Annotation From UniProt
 
Key Word From UniProt
 3D-structure; Completeproteome; DNAdamage; DNArepair; DNAreplication; Endonuclease; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Referenceproteome
Protein Sequence
 MDLADLVKDV KRELSFSELK GKRVSIDGYN ALYQFLAAIR QPDGTPLMDS QGRVTSHLSG 60
 LFYRTINILE EGVIPIYVFD GKPPEQKSEE LERRRKAKEE AERKLERAKS EGKIEELRKY 120
 SQAILRLSNI MVEESKKLLR AMGIPIVQAP SEGEAEAAYL NKLGLSWAAA SQDYDAILFG 180
 AKRLVRNLTI TGKRKLPNKD VYVEIKPELI ETEILLKKLG ITREQLIDIG ILIGTDYNPD 240
 GIRGIGPERA LKIIKKYGKI EKAMEYGEIS KKDINFNIDE IRGLFLNPQV VKPEEALDLN 300
 EPNGEDIINI LVYEHNFSEE RVKNGIERLT KAIKEAKGAS RQTGLDRWF 349
  GO:0008409   F:5'-3' exonuclease activity IEA:UniProtKB-HAMAP
  GO:0017108   F:5'-flap endonuclease activity IEA:UniProtKB-HAMAP
  GO:0003677   F:DNA binding IEA:UniProtKB-HAMAP
  GO:0000287   F:magnesium ion binding IEA:UniProtKB-HAMAP
  GO:0006281   P:DNA repair IEA:UniProtKB-HAMAP
  GO:0043137   P:DNA replication, removal of RNA primer IEA:UniProtKB-HAMAP
  IPR020045   5-3_exonuclease_C.
  IPR023426   Flap_endonuc.
  IPR019973   Flap_structure-sp_endonuc_arc.
  IPR008918   HhH2.
  IPR006086   XPG-I_dom.
  IPR006084   XPG/Rad2.
  IPR019974   XPG_CS.
  IPR006085   XPG_DNA_repair_N.
  PF00867   XPG_I.
  PF00752   XPG_N.
  SM00279   HhH2.
  SM00484   XPGI.
  SM00485   XPGN.
  PS00841   XPG_1.
  PR00853   XPGRADSUPER.