| Tag | Content |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Alanine--tRNA ligase |
Protein Synonyms/Alias | AlaRS |
Gene Name | alaS |
Gene Synonyms/Alias | CA_C1678 |
Created Date | 3-June-2014 |
Organism | Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) |
NCBI Taxa ID | 272562 |
Phosphorylation | Position | Peptide | Code | Type | References | | 375 | GMEILKNYIDELSLE | Y | HTP | [1] | | 388 | LENKKVMSGEKAFRL | S | HTP | [1] | |
Reference | [1]Phosphoproteomic investigation of a solvent producing bacterium Clostridium acetobutylicum. Bai X,Ji Z Appl. Microbiol. Biotechnol. 2012, Jul;95(1):201-11. [ PMID:22627760] |
Functional Description From UniProt | FUNCTION: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain (By similarity) |
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| Aminoacyl-tRNAsynthetase; ATP-binding; Completeproteome; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; Proteinbiosynthesis; Referenceproteome; RNA-binding; tRNA-binding; Zinc |
Protein Sequence | MKYMGLNDIR ESYLSFFEKK EHLRLPSFSL IPKNDKSLLL INAGMAPLKP YFTGLQTPPK 60
TRVTTCQKCI RTGDIENIGK TSRHGTFFEM LGNFSFGDYF KEEVISWAWE YITEVLKFPK 120
DRIYITIYLD DDEAFKIWTE KAGVDPSRIF RFGKEDNFWE HGSGPCGPCS EMHFDRREKP 180
DLIKTREKFI ELQDKDEVIE FWNLVFTQFD KDEDGNYNKL KNPNIDTGMG LERIATIMQN 240
TDSIFEIDTI REVLDAVCKI CNVKYGENHK NDVSLRIITD HIRSVTFMIS DGILPSNEGR 300
GYVLRRLLRR AARHGKTLGI EKTFLCGLCD VVIKNSKGAY KELEEKQDYI KNVIEIEEKR 360
FDETLDSGME ILKNYIDELS LENKKVMSGE KAFRLYDTYG FPVELTQEIL EEKGIEIDMN 420
DFHSEMEKQK NRARDAREES NYMGKEIKLI DKLPESVTTK FVGYNSTSTD SKVEVLIKDD 480
EFVSTINEGE SGIVVTEETP FYAEMGGQIG DKGIIFGKNG EAKVVDCKNN ISGKIIHIVQ 540
VVKGSIEKNE NVTLEVNYKK RKDICKNHTA THMLQAALKK VVGSHINQSG SYVDNERLRF 600
DFTHFTALTD EEILKVEAMV NDEIMAAYDV KTDIMSVDEA KKTGAMALFD EKYGNRVRVV 660
SVGDFSRELC GGTHVNNSGE IGLFKIISES GVAAGIRRIE AITGKEAVRY TEENDNLIRN 720
IEQELKCSKK DILNKINQYH SELKEKEKEI NILKGKLASG FEENILSSVK EVSGVKYVAS 780
EVKGISGDTL RELCDKVRNK IDDGMVLLAS KDGEKVQFVA MASKNAVKKG VHCGKVIKEV 840
ASMCGGNGGG RPDMAQAGGK DGEKLETALK EVGNIMEKLV K 881 |
| GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell GO:0004813 F:alanine-tRNA ligase activity IEA:UniProtKB-HAMAP GO:0005524 F:ATP binding IEA:UniProtKB-HAMAP GO:0000049 F:tRNA binding IEA:UniProtKB-KW GO:0008270 F:zinc ion binding IEA:UniProtKB-HAMAP GO:0006419 P:alanyl-tRNA aminoacylation IEA:UniProtKB-HAMAP |
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