| Tag | Content |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA topoisomerase 1 |
Protein Synonyms/Alias | |
Gene Name | topA |
Gene Synonyms/Alias | ECs1846, Z2536 |
Created Date | 3-June-2014 |
Organism | Escherichia coli O157:H7 |
NCBI Taxa ID | 83334 |
Phosphorylation | Position | Peptide | Code | Type | References | | 319 | YEAGYITYMRTDSTN | Y | HTP | [1] | |
Reference | [1]The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence. Hansen AM,Chaerkady R,Sharma J,Díaz-Mejía JJ,Tyagi N,Renuse S,Jacob HK,Pinto SM,Sahasrabuddhe NA,Kim MS,Delanghe B,Srinivasan N,Emili A,Kaper JB,Pandey A PLoS Pathog. 2013;9(6):e1003403. [ PMID:23785281] |
Functional Description From UniProt | FUNCTION: Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) |
| |
| ATP-binding; Completeproteome; DNA-binding; Isomerase; Magnesium; Metal-binding; Nucleotide-binding; Topoisomerase |
Protein Sequence | MGKALVIVES PAKAKTINKY LGSDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK 60
KDERGALVNR MGVDPWHNWE AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA 120
WHLREVIGGD DARYSRVVFN EITKNAIRQA FNKPGELNID RVNAQQARRF MDRVVGYMVS 180
PLLWKKIARG LSAGRVQSVA VRLVVERERE IKAFVPEEFW EVDASTTTPS GEALALQVTH 240
QNDKPFRPVN KEQTQAAVSL LEKARYSVLE REDKPTTSKP GAPFITSTLQ QAASTRLGFG 300
VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIS DNFGKKYLPE SPNQYASKEN 360
SQEAHEAIRP SDVNVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAKYDS TTLTVGAGDF 420
RLKARGRILR FDGWTKVMPA LRKGDEDRIL PAVDKGDALT LVELTPAQHF TKPPARFSEA 480
SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY 540
DFTAQMENSL DQVANHEAEW KAVLDHFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP 600
TCGRKMGIRT ASTGVFLGCS GYALPPKERC KTTINLVPEN EVLNVLEGED AETNALRAKR 660
RCPKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR IKGYDGPIVE CEKCGSEMHL 720
KMGRFGKYMA CTNEECKNTR KILRNGEVAP PKEDPVPLPE LPCEKSDAYF VLRDGAAGVF 780
LAANTFPKSR ETRAPLVEEL YRFRDRLPEK LRYLADAPQQ DPEGNKTMVR FSRKTKQQYV 840
SSEKDGKATG WSAFYVDGKW VEGKK 865 |
| GO:0005694 C:chromosome IEA:InterPro GO:0005524 F:ATP binding IEA:UniProtKB-HAMAP GO:0003677 F:DNA binding IEA:UniProtKB-KW GO:0003917 F:DNA topoisomerase type I activity IEA:UniProtKB-HAMAP GO:0003918 F:DNA topoisomerase type II (ATP-hydrolyzing) activity IEA:InterPro GO:0000287 F:magnesium ion binding IEA:UniProtKB-HAMAP GO:0006265 P:DNA topological change IEA:UniProtKB-HAMAP |
| |
| |
| |
| |
| |
