| Tag | Content |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Isoleucine--tRNA ligase |
Protein Synonyms/Alias | IleRS |
Gene Name | ileS |
Gene Synonyms/Alias | Pfl01_4852 |
Created Date | 3-June-2014 |
Organism | Pseudomonas fluorescens (strain Pf0-1) |
NCBI Taxa ID | 205922 |
Phosphorylation | Position | Peptide | Code | Type | References | | 612 | SGRKMSKSLGNVIAP | S | HTP | [1] | |
Reference | [1]Ser/Thr/Tyr phosphoproteome analysis of pathogenic and non-pathogenic Pseudomonas species. Ravichandran A,Sugiyama N,Tomita M,Swarup S,Ishihama Y Proteomics 2009, May;9(10):2764-75. [ PMID:19405024] |
Functional Description From UniProt | FUNCTION: Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity) |
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| Aminoacyl-tRNAsynthetase; ATP-binding; Completeproteome; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; Proteinbiosynthesis; Zinc |
Protein Sequence | MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK FVLHDGPPYA 60
NGTIHIGHAL NKILKDMIIR SKTLSGFDAP YVPGWDCHGL PIEHKVEVTH GKNLGADKTR 120
ELCRAYATEQ IEGQKTEFIR LGVLGDFANP YKTMNFKNEA GEIRALAEIV KGGFVFKGLK 180
PVNWCFDCGS ALAEAEVEYE NKKSSTIDVA FPIADEAKLA AAFGLPSLAK PAAIVIWTTT 240
PWTIPANQAL NVHPEFTYAL VDIGDKLLVL AEELVESCLT RYGVEGTVLA TAPGSALELI 300
NFRHPFYDRL SPVYLADYVE LGAGTGVVHS APAYGVDDFV TCKKYGMVND DILNPVQSNG 360
VYVPSLEFFG GQFIWKANPA IVDKLTEVGA LLHTTVIEHS YMHCWRHKTP LIYRATAQWF 420
IGMDKEPVSG DTLRVRSLKA IEDTKFVPAW GQARLHSMIA NRPDWCISRQ RNWGVPIPFF 480
LNKESGELHP RTVELMEEVA KRVEVEGIEA WFKLDAAELL GDEAPQYDKI SDTLDVWFDS 540
GTTHWHVLRG SHPMGHETGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF 600
TVDESGRKMS KSLGNVIAPQ KVNDTLGADI MRLWVASTDY SGEMAVSEQI LQRSADAYRR 660
IRNTARFLLS NLTGFNPATD LLPAEDMLAL DRWAVDRTLL LQRELQEHYG EYRFWNVYSK 720
IHNFCVQELG GFYLDIIKDR QYTTGADSKA RRSCQTALFH ISEALVRWIA PILAFTADEL 780
WQYLPGERNE SVMLNTWYEG LTELPEGFEL GREYWDRIME VKVAVNKEME IQRAAKAVGG 840
NLQAEVTLFA EDALTADLAK LSNELRFVLI TSTATVAPFV QAPADAVATE VSGLKLKIVK 900
SAFPKCARCW HCREDVGVNP EHPEICGRCV DNIDGAGEVR HYA 943 |
| GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell GO:0002161 F:aminoacyl-tRNA editing activity IEA:InterPro GO:0005524 F:ATP binding IEA:UniProtKB-HAMAP GO:0004822 F:isoleucine-tRNA ligase activity IEA:UniProtKB-HAMAP GO:0008270 F:zinc ion binding IEA:UniProtKB-HAMAP GO:0006428 P:isoleucyl-tRNA aminoacylation IEA:UniProtKB-HAMAP |
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