※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Surfactin synthase subunit 2
Protein Synonyms/Alias
 
Gene Name
 srfAB
Gene Synonyms/Alias
 comL, srfA2; OrderedLocusNames=BSU03490
Created Date
 3-June-2014
Organism
 Bacillus subtilis (strain 168)
NCBI Taxa ID
 224308
Phosphorylation
Position
Peptide
Code
Type
References
2040 FFDLGGHSLKGMMLI S HTP [1],UniProt
999 FFMIGGHSLKAMMMT S HTP [1],UniProt
1381 LPLTRDTSRSPLFSV S HTP [2]
1378 IEKLPLTRDTSRSPL R HTP [2]
1377 LIEKLPLTRDTSRSP T HTP [2]
1382 PLTRDTSRSPLFSVM R HTP [2]
1380 KLPLTRDTSRSPLFS T HTP [2]
Reference
 [1]The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
 Macek B,Mijakovic I,Olsen JV,Gnad F,Kumar C,Jensen PR,Mann M
 Mol. Cell Proteomics 2007, Apr;6(4):697-707. [PMID:17218307]
 [2]Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
 Elsholz AK,Turgay K,Michalik S,Hessling B,Gronau K,Oertel D,Mäder U,Bernhardt J,Becher D,Hecker M,Gerth U
 Proc. Natl. Acad. Sci. U.S.A. 2012, May, 8;109(19):7451-6. [PMID:22517742]
Functional Description From UniProt
 FUNCTION: This protein is a multifunctional enzyme able to activate and polymerize the amino acids Leu, Glu, Asp and Val Activation sites for these AA consist of individual domains
Sequence Annotation From UniProt
 MOD_RES 999 999 O-(pantetheine 4'-phosphoryl)serine
 MOD_RES 2040 2040 O-(pantetheine 4'-phosphoryl)serine
 MOD_RES 3069 3069 O-(pantetheine 4'-phosphoryl)serine (Potential)
Key Word From UniProt
 Antibioticbiosynthesis; Completeproteome; Ligase; Multifunctionalenzyme; Phosphopantetheine; Phosphoprotein; Referenceproteome; Repeat; Sporulation
Protein Sequence
 MSKKSIQKVY ALTPMQEGML YHAMLDPHSS SYFTQLELGI HGAFDLEIFE KSVNELIRSY 60
 DILRTVFVHQ QLQKPRQVVL AERKTKVHYE DISHADENRQ KEHIERYKQD VQRQGFNLAK 120
 DILFKVAVFR LAADQLYLVW SNHHIMMDGW SMGVLMKSLF QNYEALRAGR TPANGQGKPY 180
 SDYIKWLGKQ DNEEAESYWS ERLAGFEQPS VLPGRLPVKK DEYVNKEYSF TWDETLVARI 240
 QQTANLHQVT GPNLFQAVWG IVLSKYNFTD DVIFGTVVSG RPSEINGIET MAGLFINTIP 300
 VRVKVERDAA FADIFTAVQQ HAVEAERYDY VPLYEIQKRS ALDGNLLNHL VAFENYPLDQ 360
 ELENGSMEDR LGFSIKVESA FEQTSFDFNL IVYPGKTWTV KIKYNGAAFD SAFIERTAEH 420
 LTRMMEAAVD QPAAFVREYG LVGDEEQRQI VEVFNSTKAE LPEGMAVHQV FEEQAKRTPA 480
 STAVVYEGTK LTYRELNAAA NRLARKLVEH GLQKGETAAI MNDRSVETVV GMLAVLKAGA 540
 AYVPLDPALP GDRLRFMAED SSVRMVLIGN SYTGQAHQLQ VPVLTLDIGF EESEAADNLN 600
 LPSAPSDLAY IMYTSGSTGK PKGVMIEHKS ILRLVKNAGY VPVTEEDRMA QTGAVSFDAG 660
 TFEVFGALLN GAALYPVKKE TLLDAKQFAA FLREQSITTM WLTSPLFNQL AAKDAGMFGT 720
 LRHLIIGGDA LVPHIVSKVK QASPSLSLWN GYGPTENTTF STSFLIDREY GGSIPIGKPI 780
 GNSTAYIMDE QQCLQPIGAP GELCVGGIGV ARGYVNLPEL TEKQFLEDPF RPGERIYRTG 840
 DLARWLPDGN IEFLGRIDNQ VKVRGFRIEL GEIETKLNMA EHVTEAAVII RKNKADENEI 900
 CAYFTADREV AVSELRKTLS QSLPDYMVPA HLIQMDSLPL TPNGKINKKE LPAPQSEAVQ 960
 PEYAAPKTES EKKLAEIWEG ILGVKAGVTD NFFMIGGHSL KAMMMTAKIQ EHFHKEVPIK 1020
 VLFEKPTIQE LALYLEENES KEEQTFEPIR QASYQQHYPV SPAQRRMYIL NQLGQANTSY 1080
 NVPAVLLLEG EVDKDRLENA IQQLINRHEI LRTSFDMIDG EVVQTVHKNI SFQLEAAKGR 1140
 EEDAEEIIKA FVQPFELNRA PLVRSKLVQL EEKRHLLLID MHHIITDGSS TGILIGDLAK 1200
 IYQGADLELP QIHYKDYAVW HKEQTNYQKD EEYWLDVFKG ELPILDLPAD FERPAERSFA 1260
 GERVMFGLDK QITAQIKSLM AETDTTMYMF LLAAFNVLLS KYASQDDIIV GSPTAGRTHP 1320
 DLQGVPGMFV NTVALRTAPA GDKTFAQFLE EVKTASLQAF EHQSYPLEEL IEKLPLTRDT 1380
 SRSPLFSVMF NMQNMEIPSL RLGDLKISSY SMLHHVAKFD LSLEAVEREE DIGLSFDYAT 1440
 ALFKDETIRR WSRHFVNIIK AAAANPNVRL SDVDLLSSAE TAALLEERHM TQITEATFAA 1500
 LFEKQAQQTP DHSAVKAGGN LLTYRELDEQ ANQLAHHLRA QGAGNEDIVA IVMDRSAEVM 1560
 VSILGVMKAG AAFLPIDPDT PEERIRYSLE DSGAKFAVVN ERNMTAIGQY EGIIVSLDDG 1620
 KWRNESKERP SSISGSRNLA YVIYTSGTTG KPKGVQIEHR NLTNYVSWFS EEAGLTENDK 1680
 TVLLSSYAFD LGYTSMFPVL LGGGELHIVQ KETYTAPDEI AHYIKEHGIT YIKLTPSLFH 1740
 TIVNTASFAK DANFESLRLI VLGGEKIIPT DVIAFRKMYG HTEFINHYGP TEATIGAIAG 1800
 RVDLYEPDAF AKRPTIGRPI ANAGALVLNE ALKLVPPGAS GQLYITGQGL ARGYLNRPQL 1860
 TAERFVENPY SPGSLMYKTG DVVRRLSDGT LAFIGRADDQ VKIRGYRIEP KEIETVMLSL 1920
 SGIQEAVVLA VSEGGLQELC AYYTSDQDIE KAELRYQLSL TLPSHMIPAF FVQVDAIPLT 1980
 ANGKTDRNAL PKPNAAQSGG KALAAPETAL EESLCRIWQK TLGIEAIGID DNFFDLGGHS 2040
 LKGMMLIANI QAELEKSVPL KALFEQPTVR QLAAYMEASA VSGGHQVLKP ADKQDMYPLS 2100
 SAQKRMYVLN QLDRQTISYN MPSVLLMEGE LDISRLRDSL NQLVNRHESL RTSFMEANGE 2160
 PVQRIIEKAE VDLHVFEAKE DEADQKIKEF IRPFDLNDAP LIRAALLRIE AKKHLLLLDM 2220
 HHIIADGVSR GIFVKELALL YKGEQLPEPT LHYKDFAVWQ NEAEQKERMK EHEAYWMSVL 2280
 SGELPELDLP LDYARPPVQS FKGDTIRFRT GSETAKAVEK LLAETGTTLH MVLHAVFHVF 2340
 LSKISGQRDI VIGSVTAGRT NADVQDMPGM FVNTLALRME AKEQQTFAEL LELAKQTNLS 2400
 ALEHQEYPFE DLVNQLDLPR DMSRNPLFNV MVTTENPDKE QLTLQNLSIS PYEAHQGTSK 2460
 FDLTLGGFTD ENGIGLQLEY ATDLFAKETA EKWSEYVLRL LKAVADNPNQ PLSSLLLVTE 2520
 TEKQALLEAW KGKALPVPTD KTVHQLFEET VQRHKDRPAV TYNGQSWTYG ELNAKANRLA 2580
 RILMDCGISP DDRVGVLTKP SLEMSAAVLG VLKAGAAFVP IDPDYPDQRI EYILQDSGAK 2640
 LLLKQEGISV PDSYTGDVIL LDGSRTILSL PLDENDEGNP ETAVTAENLA YMIYTSGTTG 2700
 QPKGVMVEHH ALVNLCFWHH DAFSMTAEDR SAKYAGFGFD ASIWEMFPTW TIGAELHVID 2760
 EAIRLDIVRL NDYFETNGVT ITFLPTQLAE QFMELENTSL RVLLTGGDKL KRAVKKPYTL 2820
 VNNYGPTENT VVATSAEIHP EEGSLSIGRA IANTRVYILG EGNQVQPEGV AGELCVAGRG 2880
 LARGYLNRED ETAKRFVADP FVPGERMYRT GDLVKWVNGG IEYIGRIDQQ VKVRGYRIEL 2940
 SEIEVQLAQL SEVQDAAVTA VKDKGGNTAI AAYVTPETAD IEALKSTLKE TLPDYMIPAF 3000
 WVTLNELPVT ANGKVDRKAL PEPDIEAGSG EYKAPTTDME ELLAGIWQDV LGMSEVGVTD 3060
 NFFSLGGDSI KGIQMASRLN QHGWKLEMKD LFQHPTIEEL TQYVERAEGK QADQGPVEGE 3120
 VILTPIQRWF FEKNFTNKHH WNQSVMLHAK KGFDPERVEK TLQALIEHHD ALRMVYREEN 3180
 GDIVQVYKPI GESKVSFEIV DLYGSDEEML RSQIKLLANK LQSSLDLRNG PLLKAEQYRT 3240
 EAGDHLLIAV HHLVVDGVSW RILLEDFASG YMQAEKEESL VFPQKTNSFK DWAEELAAFS 3300
 QSAHLLQQAE YWSQIAAEQV SPLPKDCETE QRIVKDTSSV LCELTAEDTK HLLTDVHQPY 3360
 GTEINDILLS ALGLTMKEWT KGAKIGINLE GHGREDIIPN VNISRTVGWF TAQYPVVLDI 3420
 SDADASAVIK TVKENLRRIP DKGVGYGILR YFTETAETKG FTPEISFNYL GQFDSEVKTD 3480
 FFEPSAFDMG RQVSGESEAL YALSFSGMIR NGRFVLSCSY NEKEFERATV EEQMERFKEN 3540
 LLMLIRHCTE KEDKEFTPSD FSAEDLEMDE MGDIFDMLEE NLK 3583
  GO:0016874   F:ligase activity IEA:UniProtKB-KW
  GO:0031177   F:phosphopantetheine binding IEA:InterPro
  GO:0017000   P:antibiotic biosynthetic process IEA:UniProtKB-KW
  GO:0030435   P:sporulation resulting in formation of a cellular spore IEA:UniProtKB-KW
  IPR010071   AA_adenyl_domain.
  IPR009081   Acyl_carrier_prot-like.
  IPR025110   AMP-bd_C.
  IPR020845   AMP-binding_CS.
  IPR000873   AMP-dep_Synth/Lig.
  IPR001242   Condensatn.
  IPR010060   NRPS_synth.
  IPR020806   PKS_PP-bd.
  IPR006162   PPantetheine_attach_site.
  PF00501   AMP-binding.
  PF13193   AMP-binding_C.
  PF00668   Condensation.
  PF00550   PP-binding.
  SM00823   PKS_PP.
  PS50075   ACP_DOMAIN.
  PS00455   AMP_BINDING.
  PS00012   PHOSPHOPANTETHEINE.