UniProt Accession

 PKNB_MYCTO;  P9WI80;  L0T5F6;  P0A5S4;  P71584

Genbank Protein ID

 AE000516

Genbank Nucleotide ID

 AAK44239.1

Protein Name

 Serine/threonine-protein kinase PknB

Protein Synonyms/Alias

 

Gene Name

 pknB

Gene Synonyms/Alias

 MT0017

Created Date

 3-June-2014

Organism

 Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

NCBI Taxa ID

 83331

Phosphorylation

Reference

 [1]A novel transmembrane serine/threonine protein kinase gene from a rifamycin SV-producing amycolatopsis mediterranei U32.
 Zhang W,Li L,Jiang W,Zhao G,Yang Y,Chiao J
 Eur. J. Biochem. 2000, Jun;267(12):3744-52. [PMID:10848993]
 [2]Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases.
 Young TA,Delagoutte B,Endrizzi JA,Falick AM,Alber T
 Nat. Struct. Biol. 2003, Mar;10(3):168-74. [PMID:12548283]
 [3]Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases.
 Durán R,Villarino A,Bellinzoni M,Wehenkel A,Fernandez P,Boitel B,Cole ST,Alzari PM,Cerveñansky C
 Biochem. Biophys. Res. Commun. 2005, Aug, 5;333(3):858-67. [PMID:15967413]
 [4]Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases.
 Prisic S,Dankwa S,Schwartz D,Chou MF,Locasale JW,Kang CM,Bemis G,Church GM,Steen H,Husson RN
 Proc. Natl. Acad. Sci. U.S.A. 2010, Apr, 20;107(16):7521-6. [PMID:20368441]
 [5]PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis.
 Boitel B,Ortiz-Lombardía M,Durán R,Pompeo F,Cole ST,Cerveñansky C,Alzari PM
 Mol. Microbiol. 2003, Sep;49(6):1493-508. [PMID:12950916]
 [6]The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape.
 Kang CM,Abbott DW,Park ST,Dascher CC,Cantley LC,Husson RN
 Genes Dev. 2005, Jul, 15;19(14):1692-704. [PMID:15985609]

Functional Description From UniProt

 FUNCTION: Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins (By similarity)

Sequence Annotation From UniProt

 TRANSMEM 333 353 Helical; (Potential)
 MOD_RES 166 166 Phosphoserine; by autocatalysis (By similarity)
 MOD_RES 169 169 Phosphoserine; by autocatalysis (By similarity)
 MOD_RES 171 171 Phosphothreonine; by autocatalysis (By similarity)
 MOD_RES 173 173 Phosphothreonine; by autocatalysis (By similarity)
 MOD_RES 294 294 Phosphothreonine; by autocatalysis (By similarity)
 MOD_RES 295 295 Phosphoserine; by autocatalysis (By similarity)
 MOD_RES 309 309 Phosphothreonine; by autocatalysis (By similarity)

Key Word From UniProt

 ATP-binding; Cellmembrane; Completeproteome; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Repeat; Serine/threonine-proteinkinase; Transferase; Transmembrane; Transmembranehelix

Protein Sequence

  IPR011009   Kinase-like_dom.
  IPR005543   PASTA.
  IPR000719   Prot_kinase_dom.
  IPR017441   Protein_kinase_ATP_BS.
  IPR002290   Ser/Thr_dual-sp_kinase_dom.
  IPR008271   Ser/Thr_kinase_AS.

  PF03793   PASTA.
  PF00069   Pkinase.

  SM00740   PASTA.
  SM00220   S_TKc.

  PS51178   PASTA.
  PS00107   PROTEIN_KINASE_ATP.
  PS50011   PROTEIN_KINASE_DOM.
  PS00108   PROTEIN_KINASE_ST.