| Tag | Content |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA topoisomerase 1 |
Protein Synonyms/Alias | |
Gene Name | topA |
Gene Synonyms/Alias | MPN_261; ORFNames=MP572 |
Created Date | 3-June-2014 |
Organism | Mycoplasma pneumoniae (strain ATCC 29342 / M129) |
NCBI Taxa ID | 272634 |
Phosphorylation | Position | Peptide | Code | Type | References | | 426 | IWIRTVASLMADCKK | S | HTP | [1] | |
Reference | [1]The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases. Schmidl SR,Gronau K,Pietack N,Hecker M,Becher D,Stülke J Mol. Cell Proteomics 2010, Jun;9(6):1228-42. [ PMID:20097688] |
Functional Description From UniProt | FUNCTION: Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) |
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| ATP-binding; Completeproteome; DNA-binding; Isomerase; Magnesium; Metal-binding; Nucleotide-binding; Referenceproteome; Repeat; Topoisomerase; Zinc; Zinc-finger |
Protein Sequence | MSKNLVVIES PNKVKTLQKY LPNDFEIVST IGHIREMVHK NFGFNEADYS PVWEDWTKSK 60
KKFSSLSFKG NLKGKKLLSK YDIIKSIKEK ASKATNIYLA TDPDREGEAI SWHVYDVLDE 120
KDKSKCQRIT FNEITKNAVL DALKNPREID QSWVQSQFAR QILDRMIGFR LSRLLNNYLS 180
AKSAGRVQSV ALRFLEEREQ EIRSFVPRFW WTLDVLLNPK AEGVREACAN RSIPIVLREI 240
NPALRAGLKF EEEKSVSGID FLDEASAKKF GEQLKGVFEV YNIDETKHYS SSPNSAYTTA 300
SLQKDAINKL GWSSKKVTLI AQHLYEGVSI NGEQTALISY PRTDSTRLSA QFQQSCKEYI 360
LNHYGEKYLS NRIVSAKGKK GEKIIQDAHE AIHPTDINIT PEMVKNAIKK DEFLLYRLIW 420
IRTVASLMAD CKKSHTHIRF INDGNKFYAS SKSLVFDGYR KIYEHFENKE SNDLYIDLDK 480
IRVGDRFMAK DIKITARQTH PAARYTQASL IEALEKSNIG RPSTYNTMAS VNLDRGYASL 540
NKHAFHVTQL GEQVNEELSK HFGKIINKEF TKNMEKSLDE IAENKKNYQE FLRDFWSNFK 600
EEVKLAEGSI QRVKKEKEFV GRDCPSCASP LLYRYTKRGN EKFVGCSNFP NCKYNEFSQN 660
KPNLTLEKLE ELCPECNSQL VKRRTKFNPN KTFVGCSNFP RCRYIKKDNA S 711 |
| GO:0005694 C:chromosome IEA:InterPro GO:0005524 F:ATP binding IEA:UniProtKB-HAMAP GO:0003677 F:DNA binding IEA:UniProtKB-KW GO:0003917 F:DNA topoisomerase type I activity IEA:UniProtKB-HAMAP GO:0000287 F:magnesium ion binding IEA:UniProtKB-HAMAP GO:0006265 P:DNA topological change IEA:UniProtKB-HAMAP |
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