UniProt Accession

 NADR_ECOLI;  P27278;  P76819;  Q2M5S7

Genbank Protein ID

 U14003;  U00096;  AP009048;  X63155

Genbank Nucleotide ID

 AAA97286.1;  AAC77343.2;  BAE78379.1

Protein Name

 Trifunctional NAD biosynthesis/regulator protein NadR

Protein Synonyms/Alias

 NMN adenylyltransferase;  NMN-AT;  NMNAT;  RNK;  NRK;  NmR-K

Gene Name

 nadR

Gene Synonyms/Alias

 nadI; OrderedLocusNames=b4390, JW5800

Created Date

 3-June-2014

Organism

 Escherichia coli (strain K12)

NCBI Taxa ID

 83333

Phosphorylation

Reference

 [1]The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence.
 Hansen AM,Chaerkady R,Sharma J,Díaz-Mejía JJ,Tyagi N,Renuse S,Jacob HK,Pinto SM,Sahasrabuddhe NA,Kim MS,Delanghe B,Srinivasan N,Emili A,Kaper JB,Pandey A
 PLoS Pathog. 2013;9(6):e1003403. [PMID:23785281]

Functional Description From UniProt

 FUNCTION: This enzyme has three activities: DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase within the cell, the affinity of NadR for the nadB operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes. The RN kinase activity catalyzes the phosphorylation of RN to form nicotinamide ribonucleotide. The NMN adenylyltransferase activity catalyzes the transfer of the AMP moiety of ATP to nicotinamide ribonucleotide to form NAD(+). The NMN adenylyltransferase domain also functions as the NAD and ATP sensor

Sequence Annotation From UniProt

 

Key Word From UniProt

 ATP-binding; Cellmembrane; Completeproteome; Cytoplasm; DNA-binding; Kinase; Membrane; Multifunctionalenzyme; NAD; Nucleotide-binding; Pyridinenucleotidebiosynthesis; Referenceproteome; Repressor; Transcription; Transcriptionregulation; Transferase

Protein Sequence

  GO:0005737   C:cytoplasm IEA:UniProtKB-SubCell
  GO:0005886   C:plasma membrane IEA:UniProtKB-SubCell
  GO:0005524   F:ATP binding IEA:UniProtKB-KW
  GO:0000986   F:bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding IDA:EcoCyc
  GO:0000287   F:magnesium ion binding IDA:EcoCyc
  GO:0000309   F:nicotinamide-nucleotide adenylyltransferase activity IDA:EcoCyc
  GO:0050262   F:ribosylnicotinamide kinase activity IEA:UniProtKB-EC
  GO:0009435   P:NAD biosynthetic process IEA:UniProtKB-UniPathway
  GO:0045892   P:negative regulation of transcription, DNA-templated IEA:InterPro
  GO:0006351   P:transcription, DNA-templated IEA:UniProtKB-KW
  GO:0006810   P:transport IEA:InterPro

  IPR016429   Bifunc_transcrip_reg_NadR.
  IPR001387   Cro/C1-type_HTH.
  IPR004821   Cyt_trans-like.
  IPR010982   Lambda_DNA-bd_dom.
  IPR006417   NadR_NMN_Atrans.
  IPR027417   P-loop_NTPase.
  IPR014729   Rossmann-like_a/b/a_fold.

  PF01381   HTH_3.

  SM00530   HTH_XRE.

  PS50943   HTH_CROC1.