※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Asparagine synthetase B [glutamine-hydrolyzing]
Protein Synonyms/Alias
 AS-B
Gene Name
 asnB
Gene Synonyms/Alias
 b0674, JW0660
Created Date
 3-June-2014
Organism
 Escherichia coli (strain K12)
NCBI Taxa ID
 83333
Phosphorylation
Position
Peptide
Code
Type
References
440 LRECFEAYLPASVAW Y HTP [1]
314 DAIRDVIYHIETYDV Y HTP [1]
Reference
 [1]The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence.
 Hansen AM,Chaerkady R,Sharma J,Díaz-Mejía JJ,Tyagi N,Renuse S,Jacob HK,Pinto SM,Sahasrabuddhe NA,Kim MS,Delanghe B,Srinivasan N,Emili A,Kaper JB,Pandey A
 PLoS Pathog. 2013;9(6):e1003403. [PMID:23785281]
Functional Description From UniProt
 FUNCTION: Catalyzes the ATP-dependent conversion of aspartate into asparagine, using glutamine as a source of nitrogen. Can also use ammonia as the nitrogen source in vitro, albeit with lower efficiency. As nucleotide substrates, ATP and dATP are utilized at a similar rate in both the glutamine- and ammonia-dependent reactions, whereas GTP utilization is only 15% that of ATP, and CTP, UTP, ITP and XTP are very poor or not substrates. Also exhibits glutaminase activity
Sequence Annotation From UniProt
 
Key Word From UniProt
 3D-structure; Amino-acidbiosynthesis; Asparaginebiosynthesis; ATP-binding; Completeproteome; Directproteinsequencing; Glutamineamidotransferase; Ligase; Nucleotide-binding; Referenceproteome
Protein Sequence
 MCSIFGVFDI KTDAVELRKK ALELSRLMRH RGPDWSGIYA SDNAILAHER LSIVDVNAGA 60
 QPLYNQQKTH VLAVNGEIYN HQALRAEYGD RYQFQTGSDC EVILALYQEK GPEFLDDLQG 120
 MFAFALYDSE KDAYLIGRDH LGIIPLYMGY DEHGQLYVAS EMKALVPVCR TIKEFPAGSY 180
 LWSQDGEIRS YYHRDWFDYD AVKDNVTDKN ELRQALEDSV KSHLMSDVPY GVLLSGGLDS 240
 SIISAITKKY AARRVEDQER SEAWWPQLHS FAVGLPGSPD LKAAQEVANH LGTVHHEIHF 300
 TVQEGLDAIR DVIYHIETYD VTTIRASTPM YLMSRKIKAM GIKMVLSGEG SDEVFGGYLY 360
 FHKAPNAKEL HEETVRKLLA LHMYDCARAN KAMSAWGVEA RVPFLDKKFL DVAMRINPQD 420
 KMCGNGKMEK HILRECFEAY LPASVAWRQK EQFSDGVGYS WIDTLKEVAA QQVSDQQLET 480
 ARFRFPYNTP TSKEAYLYRE IFEELFPLPS AAECVPGGPS VACSSAKAIE WDEAFKKMDD 540
 PSGRAVGVHQ SAYK 554
  GO:0005737   C:cytoplasm IDA:EcoliWiki
  GO:0016597   F:amino acid binding IDA:UniProtKB
  GO:0004066   F:asparagine synthase (glutamine-hydrolyzing) activity IDA:UniProtKB
  GO:0004071   F:aspartate-ammonia ligase activity IDA:UniProtKB
  GO:0005524   F:ATP binding IDA:UniProtKB
  GO:0042802   F:identical protein binding IPI:IntAct
  GO:0006529   P:asparagine biosynthetic process IDA:UniProtKB
  GO:0008652   P:cellular amino acid biosynthetic process IMP:EcoliWiki
  GO:0009063   P:cellular amino acid catabolic process IMP:EcoliWiki
  GO:0006541   P:glutamine metabolic process IMP:EcoliWiki
  GO:0070981   P:L-asparagine biosynthetic process IEA:UniProtKB-UniPathway
  IPR006426   Asn_synth_AEB.
  IPR001962   Asn_synthase.
  IPR017932   GATase_2_dom.
  IPR000583   GATase_dom.
  IPR014729   Rossmann-like_a/b/a_fold.
  PF00733   Asn_synthase.
  PF13537   GATase_7.
  PS51278   GATASE_TYPE_2.