※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Levansucrase and sucrase synthesis operon antiterminator
Protein Synonyms/Alias
 
Gene Name
 sacY
Gene Synonyms/Alias
 sacS; OrderedLocusNames=BSU38420
Created Date
 3-June-2014
Organism
 Bacillus subtilis (strain 168)
NCBI Taxa ID
 224308
Phosphorylation
Position
Peptide
Code
Type
References
207 SYERLVTHLRFAIQH H LTP [1],UniProt
269 ELCYIAMHIQRFYQR H LTP [1],UniProt
99 IHVAFSDHLSFAIER H LTP [2],UniProt
Reference
 [1]Multiple phosphorylation of SacY, a Bacillus subtilis transcriptional antiterminator negatively controlled by the phosphotransferase system.
 Tortosa P,Aymerich S,Lindner C,Saier MH,Reizer J,Le Coq D
 J. Biol. Chem. 1997, Jul, 4;272(27):17230-7. [PMID:9202047]
 [2]Sites of positive and negative regulation in the Bacillus subtilis antiterminators LicT and SacY.
 Tortosa P,Declerck N,Dutartre H,Lindner C,Deutscher J,Le Coq D
 Mol. Microbiol. 2001, Sep;41(6):1381-93. [PMID:11580842]
Functional Description From UniProt
 FUNCTION: In the presence of sucrose, SacY is activated and prevents premature termination of transcription by binding to a RNA-antiterminator (RAT) sequence (partially overlapping with the terminator sequence) located upstream of the sacB gene. Formation of the SacY-RAT complex prevents alternative formation of the terminator, allowing transcription of the sacB gene. In the absence of sucrose, inhibition of SacY activity by SacX leads to termination of transcription
Sequence Annotation From UniProt
 MOD_RES 99 99 Phosphohistidine; by HPr
 MOD_RES 207 207 Phosphohistidine; by HPr
 MOD_RES 269 269 Phosphohistidine; by HPr
Key Word From UniProt
 3D-structure; Activator; Completeproteome; Phosphoprotein; Referenceproteome; Repeat; RNA-binding; Transcription; Transcriptionregulation
Protein Sequence
 MKIKRILNHN AIVVKDQNEE KILLGAGIAF NKKKNDIVDP SKIEKTFIRK DTPDYKQFEE 60
 ILETLPEDHI QISEQIISHA EKELNIKINE RIHVAFSDHL SFAIERLSNG MVIKNPLLNE 120
 IKVLYPKEFQ IGLWARALIK DKLGIHIPDD EIGNIAMHIH TARNNAGDMT QTLDITTMIR 180
 DIIEIIEIQL SINIVEDTIS YERLVTHLRF AIQHIKAGES IYELDAEMID IIKEKFKDAF 240
 LCALSIGTFV KKEYGFEFPE KELCYIAMHI QRFYQRSVAR 280
  GO:0003723   F:RNA binding IEA:UniProtKB-KW
  GO:0045893   P:positive regulation of transcription, DNA-templated IEA:InterPro
  GO:0006351   P:transcription, DNA-templated IEA:UniProtKB-KW
  IPR004341   CAT_RNA-bd_dom.
  IPR011608   PRD.
  IPR001550   Transcrpt_antitermin_CS.
  PF03123   CAT_RBD.
  PF00874   PRD.
  SM01061   CAT_RBD.
  PS00654   PRD_1.
  PS51372   PRD_2.