※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 50S ribosomal protein L14
Protein Synonyms/Alias
 
Gene Name
 rplN
Gene Synonyms/Alias
 b3310, JW3272
Created Date
 3-June-2014
Organism
 Escherichia coli (strain K12)
NCBI Taxa ID
 83333
Phosphorylation
Position
Peptide
Code
Type
References
109 PVTRELRSEKFMKII S HTP [1]
14 MLNVADNSGARRVMC S HTP [1]
42 VGDIIKITIKEAIPR T HTP [2]
32 LGGSHRRYAGVGDII Y HTP [2]
Reference
 [1]Comprehensive analysis of phosphorylated proteins of Escherichia coli ribosomes.
 Soung GY,Miller JL,Koc H,Koc EC
 J. Proteome Res. 2009, Jul;8(7):3390-402. [PMID:19469554]
 [2]The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence.
 Hansen AM,Chaerkady R,Sharma J,Díaz-Mejía JJ,Tyagi N,Renuse S,Jacob HK,Pinto SM,Sahasrabuddhe NA,Kim MS,Delanghe B,Srinivasan N,Emili A,Kaper JB,Pandey A
 PLoS Pathog. 2013;9(6):e1003403. [PMID:23785281]
Functional Description From UniProt
 FUNCTION: This protein binds directly to 23S ribosomal RNA. In the E.coli 70S ribosome (PubMed:12809609) it has been modeled to make two contacts with the 16S rRNA of the 30S subunit, forming part of bridges B5 and B8, connecting the 2 subunits. Although the protein undergoes significant rotation during the transition from an initiation to and EF-G bound state, the bridges remain stable. In the 3.5 A resolved structures (PubMed:16272117) L14 and L19 interact and together make contact with the 16S rRNA in bridges B5 and B8
Sequence Annotation From UniProt
 
Key Word From UniProt
 3D-structure; Completeproteome; Directproteinsequencing; Referenceproteome; Ribonucleoprotein; Ribosomalprotein; RNA-binding; rRNA-binding
Protein Sequence
 MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA 60
 VVVRTKKGVR RPDGSVIRFD GNACVLLNNN SEQPIGTRIF GPVTRELRSE KFMKIISLAP 120
 EVL 123
  GO:0022625   C:cytosolic large ribosomal subunit IDA:EcoCyc
  GO:0070180   F:LSU rRNA binding IDA:EcoCyc
  GO:0003735   F:structural constituent of ribosome IEA:InterPro
  GO:0006412   P:translation IEA:UniProtKB-HAMAP
  IPR005745   Ribosomal_L14_bac-type.
  IPR019972   Ribosomal_L14_CS.
  IPR023571   Ribosomal_L14_dom.
  IPR000218   Ribosomal_L14b/L23e.
  PF00238   Ribosomal_L14.
  PS00049   RIBOSOMAL_L14.