※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine hydroxymethyltransferase
Protein Synonyms/Alias
 SHMT;  Serine methylase
Gene Name
 glyA
Gene Synonyms/Alias
 b2551, JW2535
Created Date
 3-June-2014
Organism
 Escherichia coli (strain K12)
NCBI Taxa ID
 83333
Phosphorylation
Position
Peptide
Code
Type
References
55 GSQLTNKYAEGYPGK Y HTP [1]
59 TNKYAEGYPGKRYYG Y HTP [2]
Reference
 [1]Global dynamics of the Escherichia coli proteome and phosphoproteome during growth in minimal medium.
 Soares NC,Spät P,Krug K,Macek B
 J. Proteome Res. 2013, Jun, 7;12(6):2611-21. [PMID:23590516]
 [2]The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence.
 Hansen AM,Chaerkady R,Sharma J,Díaz-Mejía JJ,Tyagi N,Renuse S,Jacob HK,Pinto SM,Sahasrabuddhe NA,Kim MS,Delanghe B,Srinivasan N,Emili A,Kaper JB,Pandey A
 PLoS Pathog. 2013;9(6):e1003403. [PMID:23785281]
Functional Description From UniProt
 FUNCTION: Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism Thus, is able to catalyze the cleavage of allothreonine and 3- phenylserine. Also catalyzes the irreversible conversion of 5,10- methenyltetrahydrofolate to 5-formyltetrahydrofolate
Sequence Annotation From UniProt
 MOD_RES 54 54 N6-acetyllysine
 MOD_RES 62 62 N6-succinyllysine
 MOD_RES 229 229 N6-(pyridoxal phosphate)lysine
 MOD_RES 242 242 N6-succinyllysine
 MOD_RES 250 250 N6-acetyllysine; alternate
 MOD_RES 250 250 N6-succinyllysine; alternate
 MOD_RES 277 277 N6-succinyllysine
 MOD_RES 285 285 N6-acetyllysine
 MOD_RES 293 293 N6-succinyllysine
 MOD_RES 331 331 N6-succinyllysine
 MOD_RES 346 346 N6-succinyllysine
 MOD_RES 354 354 N6-acetyllysine; alternate
 MOD_RES 354 354 N6-succinyllysine; alternate
 MOD_RES 375 375 N6-acetyllysine
Key Word From UniProt
 3D-structure; Acetylation; Amino-acidbiosynthesis; Completeproteome; Cytoplasm; Directproteinsequencing; One-carbonmetabolism; Pyridoxalphosphate; Referenceproteome; Transferase
Protein Sequence
 MLKREMNIAD YDAELWQAME QEKVRQEEHI ELIASENYTS PRVMQAQGSQ LTNKYAEGYP 60
 GKRYYGGCEY VDIVEQLAID RAKELFGADY ANVQPHSGSQ ANFAVYTALL EPGDTVLGMN 120
 LAHGGHLTHG SPVNFSGKLY NIVPYGIDAT GHIDYADLEK QAKEHKPKMI IGGFSAYSGV 180
 VDWAKMREIA DSIGAYLFVD MAHVAGLVAA GVYPNPVPHA HVVTTTTHKT LAGPRGGLIL 240
 AKGGSEELYK KLNSAVFPGG QGGPLMHVIA GKAVALKEAM EPEFKTYQQQ VAKNAKAMVE 300
 VFLERGYKVV SGGTDNHLFL VDLVDKNLTG KEADAALGRA NITVNKNSVP NDPKSPFVTS 360
 GIRVGTPAIT RRGFKEAEAK ELAGWMCDVL DSINDEAVIE RIKGKVLDIC ARYPVYA 417
  GO:0005829   C:cytosol IDA:UniProtKB
  GO:0016020   C:membrane IDA:UniProtKB
  GO:0004372   F:glycine hydroxymethyltransferase activity IDA:EcoCyc
  GO:0042802   F:identical protein binding IPI:IntAct
  GO:0030170   F:pyridoxal phosphate binding IDA:EcoCyc
  GO:0008270   F:zinc ion binding IDA:EcoliWiki
  GO:0019264   P:glycine biosynthetic process from serine IDA:EcoCyc
  GO:0006546   P:glycine catabolic process IMP:EcoCyc
  GO:0006565   P:L-serine catabolic process IDA:EcoCyc
  GO:0035999   P:tetrahydrofolate interconversion IEA:UniProtKB-UniPathway
  IPR015424   PyrdxlP-dep_Trfase.
  IPR015421   PyrdxlP-dep_Trfase_major_sub1.
  IPR015422   PyrdxlP-dep_Trfase_major_sub2.
  IPR001085   Ser_HO-MeTrfase.
  IPR019798   Ser_HO-MeTrfase_PLP_BS.
  PF00464   SHMT.
  PS00096   SHMT.