※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-adenosylmethionine synthase
Protein Synonyms/Alias
 AdoMet synthase
Gene Name
 metK
Gene Synonyms/Alias
 metX; OrderedLocusNames=b2942, JW2909
Created Date
 3-June-2014
Organism
 Escherichia coli (strain K12)
NCBI Taxa ID
 83333
Phosphorylation
Position
Peptide
Code
Type
References
198 SEEIDQKSLQEAVME S HTP [1]
Reference
 [1]Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation.
 Macek B,Gnad F,Soufi B,Kumar C,Olsen JV,Mijakovic I,Mann M
 Mol. Cell Proteomics 2008, Feb;7(2):299-307. [PMID:17938405]
Functional Description From UniProt
 FUNCTION: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. Is essential for growth
Sequence Annotation From UniProt
 MOD_RES 3 3 N6-acetyllysine
Key Word From UniProt
 3D-structure; Acetylation; ATP-binding; Cobalt; Completeproteome; Cytoplasm; Magnesium; Metal-binding; Nucleotide-binding; One-carbonmetabolism; Potassium; Referenceproteome; Transferase
Protein Sequence
 MAKHLFTSES VSEGHPDKIA DQISDAVLDA ILEQDPKARV ACETYVKTGM VLVGGEITTS 60
 AWVDIEEITR NTVREIGYVH SDMGFDANSC AVLSAIGKQS PDINQGVDRA DPLEQGAGDQ 120
 GLMFGYATNE TDVLMPAPIT YAHRLVQRQA EVRKNGTLPW LRPDAKSQVT FQYDDGKIVG 180
 IDAVVLSTQH SEEIDQKSLQ EAVMEEIIKP ILPAEWLTSA TKFFINPTGR FVIGGPMGDC 240
 GLTGRKIIVD TYGGMARHGG GAFSGKDPSK VDRSAAYAAR YVAKNIVAAG LADRCEIQVS 300
 YAIGVAEPTS IMVETFGTEK VPSEQLTLLV REFFDLRPYG LIQMLDLLHP IYKETAAYGH 360
 FGREHFPWEK TDKAQLLRDA AGLK 384
  GO:0005829   C:cytosol IDA:UniProtKB
  GO:0005524   F:ATP binding IEA:UniProtKB-HAMAP
  GO:0042802   F:identical protein binding IPI:IntAct
  GO:0000287   F:magnesium ion binding IDA:EcoCyc
  GO:0004478   F:methionine adenosyltransferase activity IDA:EcoCyc
  GO:0030955   F:potassium ion binding IDA:EcoCyc
  GO:0006730   P:one-carbon metabolic process IEA:UniProtKB-HAMAP
  GO:0006556   P:S-adenosylmethionine biosynthetic process IMP:EcoCyc
  IPR022631   ADOMET_SYNTHASE_CS.
  IPR022630   S-AdoMet_synt_C.
  IPR022629   S-AdoMet_synt_central.
  IPR022628   S-AdoMet_synt_N.
  IPR002133   S-AdoMet_synthetase.
  IPR022636   S-AdoMet_synthetase_sfam.
  PF02773   S-AdoMet_synt_C.
  PF02772   S-AdoMet_synt_M.
  PF00438   S-AdoMet_synt_N.
  PS00376   ADOMET_SYNTHASE_1.
  PS00377   ADOMET_SYNTHASE_2.