※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peptide chain release factor 3
Protein Synonyms/Alias
 RF-3
Gene Name
 prfC
Gene Synonyms/Alias
 miaD, tos; OrderedLocusNames=b4375, JW5873
Created Date
 3-June-2014
Organism
 Escherichia coli (strain K12)
NCBI Taxa ID
 83333
Phosphorylation
Position
Peptide
Code
Type
References
195 DETYLYQSGKGHTIQ S HTP [1]
Reference
 [1]Global dynamics of the Escherichia coli proteome and phosphoproteome during growth in minimal medium.
 Soares NC,Spät P,Krug K,Macek B
 J. Proteome Res. 2013, Jun, 7;12(6):2611-21. [PMID:23590516]
Functional Description From UniProt
 FUNCTION: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons It may interact directly with the ribosome. The stimulation of RF- 1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP
Sequence Annotation From UniProt
 
Key Word From UniProt
 3D-structure; Completeproteome; Cytoplasm; Directproteinsequencing; GTP-binding; Nucleotide-binding; Proteinbiosynthesis; Referenceproteome
Protein Sequence
 MTLSPYLQEV AKRRTFAIIS HPDAGKTTIT EKVLLFGQAI QTAGTVKGRG SNQHAKSDWM 60
 EMEKQRGISI TTSVMQFPYH DCLVNLLDTP GHEDFSEDTY RTLTAVDCCL MVIDAAKGVE 120
 DRTRKLMEVT RLRDTPILTF MNKLDRDIRD PMELLDEVEN ELKIGCAPIT WPIGCGKLFK 180
 GVYHLYKDET YLYQSGKGHT IQEVRIVKGL NNPDLDAAVG EDLAQQLRDE LELVKGASNE 240
 FDKELFLAGE ITPVFFGTAL GNFGVDHMLD GLVEWAPAPM PRQTDTRTVE ASEDKFTGFV 300
 FKIQANMDPK HRDRVAFMRV VSGKYEKGMK LRQVRTAKDV VISDALTFMA GDRSHVEEAY 360
 PGDILGLHNH GTIQIGDTFT QGEMMKFTGI PNFAPELFRR IRLKDPLKQK QLLKGLVQLS 420
 EEGAVQVFRP ISNNDLIVGA VGVLQFDVVV ARLKSEYNVE AVYESVNVAT ARWVECADAK 480
 KFEEFKRKNE SQLALDGGDN LAYIATSMVN LRLAQERYPD VQFHQTREH 529
  GO:0005737   C:cytoplasm IEA:UniProtKB-SubCell
  GO:0019003   F:GDP binding IDA:EcoCyc
  GO:0005525   F:GTP binding IEA:UniProtKB-HAMAP
  GO:0003924   F:GTPase activity IDA:EcoCyc
  GO:0016150   F:translation release factor activity, codon nonspecific IDA:EcoCyc
  GO:0016149   F:translation release factor activity, codon specific IEA:UniProtKB-HAMAP
  GO:0006184   P:GTP catabolic process IDA:GOC
  GO:0006449   P:regulation of translational termination IEA:UniProtKB-HAMAP
  GO:0006415   P:translational termination IDA:EcoCyc
  IPR000795   EF_GTP-bd_dom.
  IPR009022   EFG_III-V.
  IPR027417   P-loop_NTPase.
  IPR004548   PrfC.
  IPR005225   Small_GTP-bd_dom.
  IPR009000   Transl_B-barrel.
  IPR004161   Transl_elong_EFTu/EF1A_2.
  PF00009   GTP_EFTU.
  PF03144   GTP_EFTU_D2.
  PS00301   EFACTOR_GTP.
  PR00315   ELONGATNFCT.