※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chaperone protein DnaK
Protein Synonyms/Alias
 
Gene Name
 dnaK
Gene Synonyms/Alias
 groP, grpF, seg; OrderedLocusNames=b0014, JW0013
Created Date
 3-June-2014
Organism
 Escherichia coli (strain K12)
NCBI Taxa ID
 83333
Phosphorylation
Position
Peptide
Code
Type
References
199 VYDLGGGTFDISIIE T LTP [1],UniProt
453 KRAADNKSLGQFNLD S HTP [2]
611 QQHAQQQTAGADASA T HTP [3]
617 QTAGADASANNAKDD S HTP [3]
504 QKITIKASSGLNEDE S HTP [3]
274 EKAKIELSSAQQTDV S HTP [3]
Reference
 [1]DnaK as a thermometer: threonine-199 is site of autophosphorylation and is critical for ATPase activity.
 McCarty JS,Walker GC
 Proc. Natl. Acad. Sci. U.S.A. 1991, Nov, 1;88(21):9513-7. [PMID:1835085]
 [2]Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation.
 Macek B,Gnad F,Soufi B,Kumar C,Olsen JV,Mijakovic I,Mann M
 Mol. Cell Proteomics 2008, Feb;7(2):299-307. [PMID:17938405]
 [3]Global dynamics of the Escherichia coli proteome and phosphoproteome during growth in minimal medium.
 Soares NC,Spät P,Krug K,Macek B
 J. Proteome Res. 2013, Jun, 7;12(6):2611-21. [PMID:23590516]
Functional Description From UniProt
 FUNCTION: Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock
Sequence Annotation From UniProt
 MOD_RES 70 70 N6-succinyllysine
 MOD_RES 109 109 N6-acetyllysine
 MOD_RES 199 199 Phosphothreonine; by autocatalysis
 MOD_RES 245 245 N6-acetyllysine; alternate
 MOD_RES 245 245 N6-succinyllysine; alternate
 MOD_RES 246 246 N6-succinyllysine
 MOD_RES 304 304 N6-acetyllysine; alternate
 MOD_RES 304 304 N6-succinyllysine; alternate
 MOD_RES 359 359 N6-succinyllysine
 MOD_RES 421 421 N6-acetyllysine
 MOD_RES 502 502 N6-succinyllysine
 MOD_RES 528 528 N6-succinyllysine
 MOD_RES 556 556 N6-acetyllysine
 MOD_RES 587 587 N6-succinyllysine
Key Word From UniProt
 3D-structure; Acetylation; ATP-binding; Cellinnermembrane; Cellmembrane; Chaperone; Completeproteome; Cytoplasm; Directproteinsequencing; DNAreplication; Membrane; Nucleotide-binding; Phosphoprotein; Referenceproteome; Stressresponse
Protein Sequence
 MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT 60
 NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL 120
 KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG 180
 LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL 240
 VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV 300
 TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE 360
 PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT 420
 KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA 480
 DGILHVSAKD KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD 540
 HLLHSTRKQV EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME 600
 IAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK 638
  GO:0005737   C:cytoplasm IDA:UniProtKB
  GO:0016020   C:membrane IDA:UniProtKB
  GO:0005886   C:plasma membrane IEA:UniProtKB-SubCell
  GO:0043531   F:ADP binding IDA:EcoCyc
  GO:0005524   F:ATP binding IDA:EcoCyc
  GO:0044183   F:protein binding involved in protein folding IDA:EcoCyc
  GO:0051082   F:unfolded protein binding IDA:EcoCyc
  GO:0008270   F:zinc ion binding IDA:EcoliWiki
  GO:0034620   P:cellular response to unfolded protein IDA:EcoCyc
  GO:0070389   P:chaperone cofactor-dependent protein refolding IDA:EcoCyc
  GO:0051085   P:chaperone mediated protein folding requiring cofactor IMP:EcoCyc
  GO:0006260   P:DNA replication IEA:UniProtKB-KW
  GO:0006461   P:protein complex assembly IDA:EcoCyc
  GO:0043241   P:protein complex disassembly IDA:EcoCyc
  GO:0043335   P:protein unfolding IDA:EcoCyc
  GO:0009408   P:response to heat IDA:EcoCyc
  IPR012725   Chaperone_DnaK.
  IPR018181   Heat_shock_70_CS.
  IPR013126   Hsp_70_fam.
  PF00012   HSP70.
  PS00297   HSP70_1.
  PS00329   HSP70_2.
  PS01036   HSP70_3.
  PR00301   HEATSHOCK70.