※ Protein Information
Tag Content
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Enolase
Protein Synonyms/Alias
 
Gene Name
 eno
Gene Synonyms/Alias
 b2779, JW2750
Created Date
 3-June-2014
Organism
 Escherichia coli (strain K12)
NCBI Taxa ID
 83333
Phosphorylation
Position
Peptide
Code
Type
References
379 SGETEDATIADLAVG T HTP [1]
375 ISHRSGETEDATIAD T HTP [1]
372 TAVISHRSGETEDAT S HTP [1]
369 AGYTAVISHRSGETE S HTP [2]
322 VGDDLFVTNTKILKE T HTP [2]
364 KMAKDAGYTAVISHR Y HTP [3]
Reference
 [1]Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation.
 Macek B,Gnad F,Soufi B,Kumar C,Olsen JV,Mijakovic I,Mann M
 Mol. Cell Proteomics 2008, Feb;7(2):299-307. [PMID:17938405]
 [2]Global dynamics of the Escherichia coli proteome and phosphoproteome during growth in minimal medium.
 Soares NC,Spät P,Krug K,Macek B
 J. Proteome Res. 2013, Jun, 7;12(6):2611-21. [PMID:23590516]
 [3]The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence.
 Hansen AM,Chaerkady R,Sharma J,Díaz-Mejía JJ,Tyagi N,Renuse S,Jacob HK,Pinto SM,Sahasrabuddhe NA,Kim MS,Delanghe B,Srinivasan N,Emili A,Kaper JB,Pandey A
 PLoS Pathog. 2013;9(6):e1003403. [PMID:23785281]
Functional Description From UniProt
 FUNCTION: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. It is also a component of the RNA degradosome, a multi-enzyme complex involved in RNA processing and messenger RNA degradation. Its interaction with RNase E is important for the turnover of mRNA, in particular on transcripts encoding enzymes of energy-generating metabolic routes. Its presence in the degradosome is required for the response to excess phosphosugar. May play a regulatory role in the degradation of specific RNAs, such as ptsG mRNA, therefore linking cellular metabolic status with post-translational gene regulation
Sequence Annotation From UniProt
 MOD_RES 257 257 N6-acetyllysine
Key Word From UniProt
 3D-structure; Acetylation; Completeproteome; Cytoplasm; Cytoskeleton; Directproteinsequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Referenceproteome; Secreted
Protein Sequence
 MSKIVKIIGR EIIDSRGNPT VEAEVHLEGG FVGMAAAPSG ASTGSREALE LRDGDKSRFL 60
 GKGVTKAVAA VNGPIAQALI GKDAKDQAGI DKIMIDLDGT ENKSKFGANA ILAVSLANAK 120
 AAAAAKGMPL YEHIAELNGT PGKYSMPVPM MNIINGGEHA DNNVDIQEFM IQPVGAKTVK 180
 EAIRMGSEVF HHLAKVLKAK GMNTAVGDEG GYAPNLGSNA EALAVIAEAV KAAGYELGKD 240
 ITLAMDCAAS EFYKDGKYVL AGEGNKAFTS EEFTHFLEEL TKQYPIVSIE DGLDESDWDG 300
 FAYQTKVLGD KIQLVGDDLF VTNTKILKEG IEKGIANSIL IKFNQIGSLT ETLAAIKMAK 360
 DAGYTAVISH RSGETEDATI ADLAVGTAAG QIKTGSMSRS DRVAKYNQLI RIEEALGEKA 420
 PYNGRKEIKG QA 432
  GO:0009986   C:cell surface IEA:UniProtKB-SubCell
  GO:0005856   C:cytoskeleton IEA:UniProtKB-SubCell
  GO:0005829   C:cytosol IDA:UniProtKB
  GO:0005576   C:extracellular region IEA:UniProtKB-SubCell
  GO:0016020   C:membrane IDA:UniProtKB
  GO:0000015   C:phosphopyruvate hydratase complex IEA:InterPro
  GO:0042802   F:identical protein binding IPI:IntAct
  GO:0000287   F:magnesium ion binding IDA:EcoliWiki
  GO:0004634   F:phosphopyruvate hydratase activity IDA:EcoliWiki
  GO:0006096   P:glycolytic process IMP:EcoliWiki
  IPR000941   Enolase.
  IPR020810   Enolase_C.
  IPR020809   Enolase_CS.
  IPR020811   Enolase_N.
  PF00113   Enolase_C.
  PF03952   Enolase_N.
  PS00164   ENOLASE.
  PR00148   ENOLASE.