| Tag | Content |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Autoinducer 1 sensor kinase/phosphatase LuxN |
Protein Synonyms/Alias | |
Gene Name | luxN |
Gene Synonyms/Alias | VIBHAR_02766 |
Created Date | 3-June-2014 |
Organism | Vibrio campbellii (strain ATCC BAA-1116 / BB120) |
NCBI Taxa ID | 338187 |
Phosphorylation | Position | Peptide | Code | Type | References | | 771 | HVDLILMDVQMPVMN | D | LTP | [1],UniProt | | 471 | ALANSIAHEMRNPLA | H | LTP | [1],UniProt | |
Reference | [1]A genetic analysis of the functions of LuxN: a two-component hybrid sensor kinase that regulates quorum sensing in Vibrio harveyi. Freeman JA,Lilley BN,Bassler BL Mol. Microbiol. 2000, Jan;35(1):139-49. [ PMID:10632884] |
Functional Description From UniProt | FUNCTION: At low cell density, in the absence of AI-1 (autoinducer 1), LuxN has a kinase activity and autophosphorylates on His-471 The phosphoryl group is then transferred on Asp-771 of the response regulator domain. The phosphoryl group is transferred to LuxU, and ultimately to LuxO. At high cell density, in the presence of AI-1, the kinase activity is inactivated, and the response regulator domain has a phosphatase activity. LuxN phosphatase acts on itself. As LuxU could function to establish an equilibrium between the aspartyl-phosphate of LuxN and the aspartyl-phosphate of LuxO, LuxU transfers phosphate from LuxO to LuxN and finally phosphate is drained from the system |
| TRANSMEM 9 29 Helical; (Potential)
TRANSMEM 41 61 Helical; (Potential)
TRANSMEM 160 180 Helical; (Potential)
TRANSMEM 196 216 Helical; (Potential)
TRANSMEM 220 242 Helical; (Potential)
TRANSMEM 251 275 Helical; (Potential)
TRANSMEM 283 301 Helical; (Potential)
MOD_RES 471 471 Phosphohistidine; by autocatalysis
MOD_RES 771 771 4-aspartylphosphate |
| ATP-binding; Cellinnermembrane; Cellmembrane; Completeproteome; Hydrolase; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Proteinphosphatase; Transferase; Transmembrane; Transmembranehelix; Two-componentregulatorysystem |
Protein Sequence | MFDFSLEAIV YAKAITLLAT VAVVMMWLFY YCYRLKQKNE VIFGTHHAAY IAYSVCIIAW 60
ISSNAYFHTD LLPELGASAG MFMAKFANLA SFFAFAFAYY FSCQLAAEQR KGKVHRWQQG 120
IFVSLTVYSL FINLRPGLTV EHVDIVGPSQ FIIEFGPHTS YFFIGLVSFV VLTLVNLVAM 180
RTNSSKLTLA KTNYMIAGIL VFMLSTAVIH LGMTYFMGDF SLTWLPPALS ISEMLFVGYA 240
LLTSRFYSVK YIAYLALSVL LVCAIFVLPL GAIFIPLTES NQWLIAIPIC ALIGITWQLL 300
YKKTSRYASF LIYGDKKTPV QQILSLEEDF KLSIDDAMRR LGKLLQIPND KLRLVTSNYN 360
ETFYEEYLSS NRSVLVFDEL SEELEYKVSA KRSMKALYDK MSSNNTALVM PLFGQGKSVT 420
HLLISPHKSN NQMFSNEEIS AVQTLLTRVQ STIEADRRIR QSRALANSIA HEMRNPLAQV 480
QLQFEALKQH IENHAPVEQI TLDIENGQAA IQRGRQLIDI ILREVSDSSP EHEPIAMTSI 540
HKAVDQAVSH YGFENEKIIE RIRLPQHTDF VAKLNETLFN FVIFNLIRNA IYYFDSYPDS 600
QIEISTKTGP YENTLIFRDT GPGIDETISH KIFDDFFSYQ KSGGSGLGLG YCQRVMRSFG 660
GRIECKSKLG TFTEFHLYFP VVPNAPKADT LRTPYFNDWK QNKRSNEHKV APNVQINNQS 720
PTVLIVDDKE VQRALVQMYL NQLGVNSLQA NNGENAVEVF KANHVDLILM DVQMPVMNGF 780
DASQRIKELS PQTPIVALSG ESGERELDMI NKLMDGRLEK PTTLNALRHV LGNWLNKNTA 840
SSACEAERE 849 |
| GO:0016021 C:integral component of membrane IEA:UniProtKB-KW GO:0005886 C:plasma membrane IEA:UniProtKB-SubCell GO:0005524 F:ATP binding IEA:UniProtKB-KW GO:0004721 F:phosphoprotein phosphatase activity IEA:UniProtKB-KW GO:0000156 F:phosphorelay response regulator activity IEA:InterPro GO:0000155 F:phosphorelay sensor kinase activity IEA:InterPro |
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